IGF1 (Human) Recombinant Protein
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Specification
Product Description
Human IGF1 (P05019, 49 a.a. - 118 a.a ) partial recombinant protein expressed in Escherichia coli.
Sequence
GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA
Host
Escherichia coli
Theoretical MW (kDa)
7.7
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/ug of protein by gel clotting method
Activity
ED50 < 5.0 ng/mL, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0 × 105 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — IGF1
Entrez GeneID
3479Protein Accession#
P05019Gene Name
IGF1
Gene Alias
IGFI
Gene Description
insulin-like growth factor 1 (somatomedin C)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene
Other Designations
insulin-like growth factor 1|somatomedin C
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