Tnf (Rat) Recombinant Protein
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Specification
Product Description
Rat Tnf (P16599, 80 a.a. - 235 a.a.) partial recombinant protein expressed in Pichia pastoris.
Sequence
MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL
Host
Yeast
Theoretical MW (kDa)
17.4
Form
Lyophilized
Preparation Method
Pichia pastoris expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/μg of protein by gel clotting method
Activity
ED50 < 50.0 pg/mL, measured in a cytotoxicity assay using mouse L-929 cells in the presence of actinomycin D, corresponding to a specific activity of > 2.0 × 107 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Tnf
Entrez GeneID
24835Protein Accession#
P16599Gene Name
Tnf
Gene Alias
MGC124630, RATTNF, TNF-alpha, Tnfa
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
tumor necrosis factor superfamily member 2|tumor necrosis factor superfamily, member 2|tumor necrosis factor alpha|tumor necrosis factor superfamily, member 2|tumor necrosis factor, alpha (cachetin)|tumor necrosis factor-alpha
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