Kitl (Mouse) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Mouse Kitl (P20826, 26 a.a. - 189 a.a.) partial recombinant protein expressed in Pichia pastoris.
Sequence
MTKEICGNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWLRDMVIQLSLSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVLCMEENAPKNIKESPKRPETRSFTPEEFFSIFNRSIDAFKDFMVASDTSDCVLSSTLGPEKDSRVSVTKPFMLPPVA
Host
Yeast
Theoretical MW (kDa)
18.4
Form
Lyophilized
Preparation Method
Pichia pastoris expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/μg of protein by gel clotting method
Activity
ED50 < 10.0 ng/mL, measured by a cell proliferation assay using human TF-1 cells, corresponding to a specific activity of > 1.0 × 105 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Kitl
Entrez GeneID
17311Protein Accession#
P20826Gene Name
Kitl
Gene Alias
Clo, Con, Gb, Kitlg, Mgf, SCF, SF, SLF, Sl, Steel, contrasted
Gene Description
kit ligand
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000022753|Steel factor|cloud gray|grizzle-belly|mast cell growth factor|stem cell factor
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