FGF1 (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human FGF1 (P05230-1, 16 a.a. - 155 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Host
Escherichia coli
Theoretical MW (kDa)
15.8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.2 EU per 1 ug of protein (determined by gel clotting method)
Activity
The ED50 as determined by a cell proliferation assay of 3T3 cells is < 0.3 ng/ml, corresponding to a specific activity of > 3.3 x 106 IU/mg in the presence of 10.0 ug/ml of heparin.
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/ml
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — FGF1
Entrez GeneID
2246Protein Accession#
P05230-1Gene Name
FGF1
Gene Alias
AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1
Gene Description
fibroblast growth factor 1 (acidic)
Omim ID
131220Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000066028|OTTHUMP00000066030|OTTHUMP00000066031|OTTHUMP00000174675|endothelial cell growth factor, alpha|endothelial cell growth factor, beta|heparin-binding growth factor 1
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