TFF3 (Human) Recombinant Protein
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Specification
Product Description
Human TFF3 (Q07654, 36 a.a. - 94 a.a.) partial recombinant protein expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
6.6
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is < 10.0 ug/ml, corresponding to a specific activity of > 100.0 IU/mg.
Storage Buffer
Lyophilized from sterile distilled Water up to 0.1 - 1.0 mg/ml
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — TFF3
Entrez GeneID
7033Protein Accession#
Q07654Gene Name
TFF3
Gene Alias
HITF, ITF, TFI, hP1.B
Gene Description
trefoil factor 3 (intestinal)
Omim ID
600633Gene Ontology
HyperlinkGene Summary
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq
Other Designations
intestinal trefoil factor|secretory protein|trefoil factor 3|trefoil factor 3, HITF, human intestinal trefoil factor
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