Tnf (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Tnf (P06804, 80 a.a. - 235 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL
Host
Escherichia coli
Theoretical MW (kDa)
17.4
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The ED50 was determined by a cell proliferation assay using murineL929 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 107 IU/mg in the presence of actinomycin D.
Storage Buffer
Lyophilized from sterile distilled Water up to 0.1 - 1.0 mg/ml
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Tnf
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