VEGFA (Human) Recombinant Protein
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Specification
Product Description
Human VEGFA (P15692-4, 27 a.a. - 191 a.a.) partial recombinant protein expressed in Pichia pastoris.
Sequence
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVYVGARCCLMPWSLPGPHPCGPCSERR
Host
Yeast
Theoretical MW (kDa)
38-40, 18-20
Form
Lyophilized
Preparation Method
Pichia pastoris expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.5 EU per 1 ug of protein (determined by gel clotting method)
Activity
The ED50 was determined by the dose-dependent stimulation of proliferation of HUVEC cells is 1.0 - 5.0 ng/ml, corresponding to a specific activity of 2.0 x 105 - 1.0 x 106units/mg.
Quality Control Testing
2 ug protein in Reducing and Non-Reducing SDS PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/ml
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — VEGFA
Entrez GeneID
7422Protein Accession#
P15692-4Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
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Interactome
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