Il6 (Rat) Recombinant Protein
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Specification
Product Description
Rat Il6 (P20607-1, 25 a.a.- 211 a.a.) partial recombinant protein expressed in HEK293 cells.
Sequence
MFPTSQVRRGDFTEDTTHNRPVYTTSQVGGLITYVLREILEMRKELCNGNSDCMNSDDALSENNLKLPEIQRNDGCFQTGYNQEICLLKICSGLLEFRFYLEFVKNNLQDNKKDKARVIQSNTETLVHIFKQEIKDSYKIVLPTPTSNALLMEKLESQKEWLRTKTIQLILKALEEFLKVTMRSTRQT
Host
Human
Theoretical MW (kDa)
21 ~ 26
Form
Lyophilized
Preparation Method
Mammalian cell (HEK 293) expression system_x005F_x000D__x000D_
Purity
> 95% as analyzed by SDS-PAGE.
Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
ED50 < 3.0 pg/mL, measured in a cell proliferation assay using 7TD1cells.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Il6
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