CSF2 (Human) Recombinant Protein
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Specification
Product Description
Human CSF2 (P04141, 18 a.a. - 144 a.,a.) partial recombinant protein expressed in CHO cells.
Sequence
MAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
Host
Mammals
Theoretical MW (kDa)
22 - 28
Form
Lyophilized
Preparation Method
Mammalian cell (CHO) expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/ugof protein(gel clotting method)
Activity
ED50 < 0.2 ng/mL, measured in a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 5.0× 106 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
Competitor -
Applications
Functional Study
SDS-PAGE
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Gene Info — CSF2
Entrez GeneID
1437Protein Accession#
P04141Gene Name
CSF2
Gene Alias
GMCSF, MGC131935, MGC138897
Gene Description
colony stimulating factor 2 (granulocyte-macrophage)
Omim ID
138960Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq
Other Designations
colony stimulating factor 2|granulocyte-macrophage colony stimulating factor|molgramostin|sargramostim
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