CSF3 (Human) Recombinant Protein
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Specification
Product Description
Human CSF3 (Q8N4W3, 27 a.a. - 200 a.a.) partial recombinant protein expressed in CHO cells.
Sequence
TVQEATPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRH
Host
Mammals
Theoretical MW (kDa)
18.7
Form
Lyophilized
Preparation Method
Mammalian cell (CHO) expression system_x005F_x000D__x000D_
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/ugof protein(gel clotting method)
Activity
ED50 < 0.1 ng/mL, determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells, corresponding to a specific activity of > 1.0 × 107 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CSF3
Entrez GeneID
1440Protein Accession#
Q8N4W3Gene Name
CSF3
Gene Alias
G-CSF, GCSF, MGC45931
Gene Description
colony stimulating factor 3 (granulocyte)
Omim ID
138970Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164327|colony stimulating factor 3|filgrastim|granulocyte colony stimulating factor|lenograstim|pluripoietin
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