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Last updated: 2022/8/14

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SaCas9 2NLS Nuclease 

  • Catalog # : P7092
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  • Specification
  • Product Description:
  • Recombinant Streptococcus aureus tag-free Cas9 nuclease expressed in an Escherichia coli with both N-terminal and C-terminal nucleic localization signal (NLS).
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • ~130 kDa
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • ≥ 95% as analyzed by SDS-PAGE
    ≥ 90% as analyzed by SEC-HPLC
  • Endotoxin Level:
  • ≤ 10 EU/mg as analyzed by gel clotting method
  • Quality Control Testing:
  • Cleavage assay

    QC Testing of P7092
    A 20 uL reaction in 1xSaCas9 Nuclease Reaction Buffer containing linearized plasmid, gRNA, and SaCas9 2NLS Nuclease for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
  • Recommend Usage:
  • CRISPR Genomic editing
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM Tris, 300 mM NaCl, 0.1 mM TCEP, pH7.5 (50% glycerol).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • CRISPR Genomic Editing
  • Application Image
  • CRISPR Genomic Editing
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