SaCas9 2NLS Nuclease
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Specification
Product Description
Recombinant Streptococcus aureus tag-free Cas9 nuclease expressed in an Escherichia coli with both N-terminal and C-terminal nucleic localization signal (NLS).
Host
Escherichia coli
Theoretical MW (kDa)
~130 kDa
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
≥ 95% as analyzed by SDS-PAGE
≥ 90% as analyzed by SEC-HPLCEndotoxin Level
≤ 10 EU/mg as analyzed by gel clotting method
Quality Control Testing
Cleavage assay
A 20 uL reaction in 1xSaCas9 Nuclease Reaction Buffer containing linearized plasmid, gRNA, and SaCas9 2NLS Nuclease for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
Recommend Usage
CRISPR Genomic editing
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris, 300 mM NaCl, 0.1 mM TCEP, pH7.5 (50% glycerol).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
CRISPR Genomic Editing
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