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Last updated: 2022/7/3

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Cas12a (Cpf1) Nuclease 

  • Catalog # : P7075
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  • Specification
  • Product Description:
  • Recombinant Acidaminococcus sp. (strain BV3L6) Cas12a (cpf1) nuclease expressed in an Escherichia coli with a C-terminal nucleic localization signal (NLS) and 6x His-tag.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • ~150 kDa
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • ≥ 90% by SDS-PAGE
  • Endotoxin Level:
  • ≤ 0.2 EU/ug of the protein by gel clotting method
  • Quality Control Testing:
  • Cleavage assay

    QC Testing of P7075
    A 20 uL reaction in 1xCas12a Nuclease Reaction Buffer containing 60 ng linearized plasmid, 10 ng crRNA, and 100 ng Cas12a (Cpf1) Nuclease for 30 mins at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
  • Recommend Usage:
  • CRISPR Genomic editing
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM Tris, 300 mM NaCl, 0.5 mM DTT, pH7.4 (50% glycerol).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • CRISPR Genomic Editing
  • Application Image
  • CRISPR Genomic Editing
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