Cas12a (Cpf1) Nuclease
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
A 20 uL reaction in 1xCas12a Nuclease Reaction Buffer containing 60 ng linearized plasmid, 10 ng crRNA, and 100 ng Cas12a (Cpf1) Nuclease for 30 mins at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
-
Specification
Product Description
Recombinant Acidaminococcus sp. (strain BV3L6) Cas12a (cpf1) nuclease expressed in an Escherichia coli with a C-terminal nucleic localization signal (NLS) and 6x His-tag.
Host
Escherichia coli
Theoretical MW (kDa)
~150 kDa
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
≥ 90% by SDS-PAGE
Endotoxin Level
≤ 0.2 EU/ug of the protein by gel clotting method
Quality Control Testing
Cleavage assay
A 20 uL reaction in 1xCas12a Nuclease Reaction Buffer containing 60 ng linearized plasmid, 10 ng crRNA, and 100 ng Cas12a (Cpf1) Nuclease for 30 mins at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
Recommend Usage
CRISPR Genomic editing
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM Tris, 300 mM NaCl, 0.5 mM DTT, pH7.4 (50% glycerol).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
CRISPR Genomic Editing
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com