Products

Product Description

RNA-guided endonuclease expressed in an Escherichia coli with Cas9 nuclease from Streptococcus pyogenes with a N terminal end nuclear localization signal (NLS).

Host

Escherichia coli

Form

Liquid

Preparation Method

Escherichia coli expression system

Quality Control Testing

Cleavage assay

Lane 1: gRNA + Cas9-N-NLS
Lane 2: gRNA + Merck
Lane 3: gRNA + IDT
Lane 4: gRNA + Thermo
Lane 5: negative control
Lane 6: marker

Recommend Usage

CRISPR Genomic editing
The optimal working dilution should be determined by the end user.

Storage Buffer

In 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, pH7.4 (50% glycerol).

Storage Instruction

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.