RNA-guided endonuclease expressed in an Escherichia coli with Cas9 nuclease from Streptococcus pyogenes with a N terminal end nuclear localization signal (NLS).
Host:
Escherichia coli
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Quality Control Testing:
Cleavage assay
Lane 1: gRNA + Cas9-N-NLS Lane 2: gRNA + Merck Lane 3: gRNA + IDT Lane 4: gRNA + Thermo Lane 5: negative control Lane 6: marker
Recommend Usage:
CRISPR Genomic editing The optimal working dilution should be determined by the end user.
Storage Buffer:
In 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, pH7.4 (50% glycerol).
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.