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Last updated: 2022/7/3

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NLS-Cas9-D10A Nickase 

  • Catalog # : P7059
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  • Specification
  • Product Description:
  • Highly purified mutant protein expressed in Escherichia coli with mutated Cas9 gene from Streptococcus pyogenes.
  • Host:
  • Escherichia coli
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Quality Control Testing:
  • Cleavage assay

    QC Testing of P7059
    Cleavage products were resolved on a 1% agarose gel. Input DNA is pUC57 plasmid DNA (OC, open circular; LIN, linearized; SC, supercoiled).
  • Recommend Usage:
  • CRISPR Genomic editing
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM Tris, 300 mM NaCl, 1 mM DTT, pH7.4 (50% glycerol).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • CRISPR Genomic Editing
  • Application Image
  • CRISPR Genomic Editing
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