Products

Product Description

Highly purified mutant protein expressed in Escherichia coli with mutated Cas9 gene from Streptococcus pyogenes.

Host

Escherichia coli

Form

Liquid

Preparation Method

Escherichia coli expression system

Quality Control Testing

Cleavage assay

Cleavage products were resolved on a 1% agarose gel. Input DNA is pUC57 plasmid DNA (OC, open circular; LIN, linearized; SC, supercoiled).

Recommend Usage

CRISPR Genomic editing
The optimal working dilution should be determined by the end user.

Storage Buffer

In 10 mM Tris, 300 mM NaCl, 1 mM DTT, pH7.4 (50% glycerol).

Storage Instruction

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.