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Last updated: 2022/7/3

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NLS-Cas9-NLS nuclease 

  • Catalog # : P7055
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  • Specification
  • Product Description:
  • Recombinant Streptococcus pyogenes Cas9 (wt) protein expressed in an E. coli with both N- and C-terminal nucleic localization signal (NLS).
  • Form:
  • Liquid
  • Preparation Method:
  • E. coli expression system
  • Purity:
  • ≥ 95% by SDS-PAGE
  • Endotoxin Level:
  • < 0.1 eu/μg
  • Activity:
  • 20 nM CRISPR/Cas9-C-NLS nuclease incubated for 1 hour at 37℃ result in 90% digestion of the substrate DNA as determined by agarose gel electrophoresis.
  • Recommend Usage:
  • CRISPR Genome editing
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH 7.4
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • CRISPR Genomic Editing
  • Electrophoresis
  • Electrophoresis
  • Electrophoresis DNA cleavage assay with NLS-Cas9-NLS nuclease. Input DNA is EcoRV-linearized pUC57 plasmid DNA. (1) DNA + gRNA + 50 ng Cas9 (2) DNA +gRNA +25 ng Cas9 (3) DNA +gRNA
  • Application Image
  • CRISPR Genomic Editing
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