Cas9-N-NLS nuclease
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More Files
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Electrophoresis
Electrophoresis DNA cleavage assay with Cas9-N-NLS nuclease. Input DNA is EcoRV-linearized pUC57 plasmid DNA. (1) DNA + gRNA + 50 ng Cas9 (2) DNA +gRNA +25 ng Cas9 (3) DNA +gRNA
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Specification
Product Description
Recombinant Streptococcus pyogenes Cas9 (wt) protein expressed in an E. coli with a N-terminal nucleic localization signal (NLS).
Form
Liquid
Preparation Method
E. coli expression system
Purity
≥ 95% by SDS-PAGE
Endotoxin Level
< 0.1 eu/μg
Activity
20 nM CRISPR/Cas9-C-NLS nuclease incubated for 1 hour at 37℃ result in 90% digestion of the substrate DNA as determined by agarose gel electrophoresis.
Recommend Usage
CRISPR Genome editing
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH 7.4
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Electrophoresis
Electrophoresis DNA cleavage assay with Cas9-N-NLS nuclease. Input DNA is EcoRV-linearized pUC57 plasmid DNA. (1) DNA + gRNA + 50 ng Cas9 (2) DNA +gRNA +25 ng Cas9 (3) DNA +gRNACRISPR Genomic Editing
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