FLT3LG (Human) Recombinant Protein
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Functional Study
The ED50 for this effect is <0.8 ng/mL, measured by the induction of proliferation in BaF3 cells.
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Specification
Product Description
Human FLT3LG recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Sequence
MTQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVASNLQDEELCGGLWRLVLAQRWMERLKTVAGSKMQGLLERVNTEIHFVTKCAFQPPPSCLRFVQTNISRLLQETSEQLVALKPWITRQNFSRCLELQCQPDSSTLPPPWSPRPLEATAPTA
Host
Escherichia coli
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.1 EU per 1 ug of the protein by the LAL method.
Activity
Measured by the induction of proliferation in BaF3 cells transfected with mouse Flt-3. The ED 50 for this effect is < 0.8 ng/mL. The specific activity of recombinant human Flt-3 Ligand is > 1.5x106 IU/mg.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 8.0.
Storage Instruction
Store at -20°C, lyophilized protein is stable for 1 year.
After reconstitution with deionized water, store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
The ED50 for this effect is <0.8 ng/mL, measured by the induction of proliferation in BaF3 cells.SDS-PAGE
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Gene Info — FLT3LG
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Interactome
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Pathway
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Disease
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