TGFB1 (Human) Recombinant Protein
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Functional Study
The ED50 for this effect is <3.2 ng/mL, measured by the induction of proliferation in MCF-7 cells.
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Specification
Product Description
Human TGFB1 recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Sequence
MALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
Host
Escherichia coli
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 98% by SDS-PAGE
Endotoxin Level
< 0.1 EU per 1 ug of the protein by the LAL method.
Activity
Measured by the ability to inhibit the IL-4 dependent proliferation in HT-2 cells. The ED 50 for this effect is < 0.1 ng/mL. The specific activity of recombinant human TGF beat 1 is approximately > 5 x 107 IU/mg. Measured by the induction of proliferation in MCF-7 cells The ED 50 for this effect is < 3.2 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 8.0.
Storage Instruction
Store at -20°C, lyophilized protein is stable for 1 year.
After reconstitution with deionized water, store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
The ED50 for this effect is <3.2 ng/mL, measured by the induction of proliferation in MCF-7 cells.SDS-PAGE
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Gene Info — TGFB1
Entrez GeneID
7040Gene Name
TGFB1
Gene Alias
CED, DPD1, TGFB, TGFbeta
Gene Description
transforming growth factor, beta 1
Gene Ontology
HyperlinkGene Summary
TGFB is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA (MIM 190170) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for this peptide. TGFB1, TGFB2 (MIM 190220), and TGFB3 (MIM 190230) all function through the same receptor signaling systems.[supplied by OMIM
Other Designations
TGF-beta 1 protein|diaphyseal dysplasia 1, progressive|transforming growth factor-beta 1
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Interactome
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Pathway
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Disease
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