IL3 (Human) Recombinant Protein
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Specification
Product Description
Human IL3 recombinant protein with His tag expressed in Escherichia coli.
Sequence
MAPMTQTTSLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF
Host
Escherichia coli
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 98% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug
Activity
Measured by the induction of TF-1 cells proliferation. The ED50 for this effect is < 0.15 ng/mL. The specific activity is approximately > 1.2 x 106 IU/mg.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 8.0.
Storage Instruction
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. Avoid repeated freezing and thawing.
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Applications
Functional Study
The ED50 for this effect is <0.15 ng/mL, measured by the induction of TF-1 cells proliferation.SDS-PAGE
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Gene Info — IL3
Entrez GeneID
3562Gene Name
IL3
Gene Alias
IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF
Gene Description
interleukin 3 (colony-stimulating factor, multiple)
Omim ID
147740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq
Other Designations
P-cell stimulating factor|hematopoietic growth factor|interleukin 3|mast-cell growth factor|multilineage-colony-stimulating factor
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Interactome
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