Il5 (Mouse) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
Il5 (Mouse) Recombinant Protein (Cat # P6989) in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 3 ng/mL.
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Specification
Product Description
Mouse Il5 (NP_034688.1, 21 a.a.- 133 a.a.) partial recombinant protein with C-terminal His tag expressed in HEK293 cells.
Host
Human
Theoretical MW (kDa)
14.2
Form
Liquid
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method).
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 3 ng/mL.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
Bioactivity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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Gene Info — Il5
Entrez GeneID
16191Protein Accession#
NP_034688.1Gene Name
Il5
Gene Alias
Il-5
Gene Description
interleukin 5
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000005818
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