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Last updated: 2022/8/14

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SPP1 (Human) Recombinant Protein BioActive

  • Catalog # : P6982
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  • Specification
  • Product Description:
  • Human SPP1 (NP_001035147, 17 a.a. - 314 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 36.2
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • > 90% by SDS-PAGE
  • Endotoxin Level:
  • < 1 EU per 1 ug of protein (determined by LAL method).
  • Activity:
  • Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When cells are added to OPN coated plates 10 ug/mL. This effect is more to 40%.
  • Quality Control Testing:
  • 3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

    QC Testing of P6982
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM Tris-HCl buffer, 2 mM EDTA, pH 7.5 (1 mM DTT, 10% glycerol).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • SDS-PAGE
  • Application Image
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 6696
  • Gene Name:
  • SPP1
  • Gene Alias:
  • BNSP,BSPI,ETA-1,MGC110940,OPN
  • Gene Description:
  • secreted phosphoprotein 1
  • Other Designations:
  • SPP1/CALPHA1 fusion,bone sialoprotein I,early T-lymphocyte activation 1,osteopontin,osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein,secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
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