Products

Product Description

Human IBMP2 (NP_001191, 283 a.a. - 396 a.a.) partial recombinant protein expressed in Escherichia coli.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins

Host

Escherichia coli

Theoretical MW (kDa)

13

Form

Liquid

Preparation Method

Escherichia coli expression system

Purity

> 90% by SDS-PAGE

Endotoxin Level

< 1 EU per 1 ug of protein (determined by LAL method).

Activity

Measured by the ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is ≤ 100 ng/mL.

Quality Control Testing

3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Lane 1: reducing conditions.
Lane 2: non-reducing conditions.

Recommend Usage

Bioactivity
SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

In 10 mM Sodium Acetate, pH 3.5.

Storage Instruction

Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.

Note

Result of bioactivity analysis