PON1 (Human) Recombinant Protein
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Specification
Product Description
Human PON1 (NP_000437, 16 a.a.- 355 a.a.) partial recombinant protein with C-terminal His tag expressed in HEK293 cells.
Host
Human
Theoretical MW (kDa)
39
Form
Liquid
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method).
Activity
Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1 pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH7.5 at 37°C.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (20% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — PON1
Entrez GeneID
5444Protein Accession#
NP_000437Gene Name
PON1
Gene Alias
ESA, PON
Gene Description
paraoxonase 1
Omim ID
168820Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq
Other Designations
arylesterase B-type|esterase A|paraoxonase B-type|serum aryldiakylphosphatase
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