Mouse Thpo (NP_033405, 22 a.a.- 356 a.a.) partial recombinant protein with C-terminal His tag expressed in HEK293 cells.
Host:
Human
Theoretical MW (kDa):
36.4
Form:
Liquid
Preparation Method:
Mammalian cell (HEK293) expression system
Purity:
> 90% by SDS-PAGE
Endotoxin Level:
< 1 EU per 1 ug of protein (determined by LAL method).
Activity:
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤ 4 ng/mL.
Quality Control Testing:
3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage:
Bioactivity SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (10% glycerol).
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of bioactivity analysis
Thpo (Mouse) Recombinant Protein (Cat # P6977) stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range ≤ 4 ng/mL.