Il1rn (Mouse) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
Il1rn (Mouse) Recombinant Protein (Cat # P6973) inhibits Mouse IL-1 alpha induced cell proliferation in the D10.G4.1 mouse helper T cell. The ED50 range is less or equal to 60 ng/mL.
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Specification
Product Description
Mouse Il1rn (NP_001034790, 27 a.a. - 178 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
20
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method).
Activity
Measured by the ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/mL with Mouse IL-1 alpha.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
Bioactivity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl buffer, 0.1 M NaCl, pH 8.0 (10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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Gene Info — Il1rn
Entrez GeneID
16181Protein Accession#
NP_001034790Gene Name
Il1rn
Gene Alias
F630041P17Rik, IL-1ra
Gene Description
interleukin 1 receptor antagonist
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000012801|OTTMUSP00000012802
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