Il1a (Rat) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
Il1a (Rat) Recombinant Protein in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 20 pg/mL.
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Specification
Product Description
Rat Il1a (NP_058715, 115 a.a. - 270 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
Host
Rat
Theoretical MW (kDa)
20.2
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
> 1 EU per 1ug of protein (determined by LAL method)
Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 20 pg/ml.
Quality Control Testing
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol)
Storage Instruction
Stored at 2°C to 8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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Gene Info — Il1a
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