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Last updated: 2022/5/22

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Tnf (Mouse) Recombinant Protein BioActive

  • Catalog # : P6954
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  • Specification
  • Product Description:
  • Mouse Tnf (NP_038721, 80 a.a. - 235 a.a.) partial recombinant protein expressed in Escherichia coli.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 17.4
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • > 95% by SDS-PAGE
  • Endotoxin Level:
  • > 1 EU per 1ug of protein (determined by LAL method)
  • Activity:
  • Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.06 ng/ml.
  • Quality Control Testing:
  • 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

    QC Testing of P6954
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4
  • Storage Instruction:
  • Stored at 2°C to 8°C for 1 week.
    For long term storage, aliquot and store at -20°C to -80°C.
    Avoid repeated freezing and thawing cycles.
  • Note:
  • Result of bioactivity analysis
  • Tnf (Mouse) Recombinant Protein stimulates cell cytotoxicity in the L929 mouse fibrosarcoma cell. The ED50 range ≤ 0.06ng/mL.


  • Applications
  • SDS-PAGE
  • Application Image
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • Tnf
  • Gene Alias:
  • DIF,MGC151434,TNF-alpha,TNFSF2,TNFalpha,Tnfa,Tnfsf1a
  • Gene Description:
  • tumor necrosis factor
  • Other Designations:
  • TNF alpha,tumor necrosis factor alpha,tumor necrosis factor-alpha
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