TPI1 (Human) Recombinant Protein
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Specification
Product Description
Human TPI1 (NP_000356, 1 - 249 a.a.) full length recombinant protein with His tag expressed in Escherichia coli expression system.
Host
Escherichia coli
Theoretical MW (kDa)
28.8
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU/ug
Activity
Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25°C.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
3 ug SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, 1 mM DTT, pH 8.0 (10% glycerol)
Storage Instruction
Store at 4°C for one week. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — TPI1
Entrez GeneID
7167Protein Accession#
P60174Gene Name
TPI1
Gene Alias
MGC88108, TPI
Gene Description
triosephosphate isomerase 1
Omim ID
190450Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
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