CSTB (Human) Recombinant Protein
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Specification
Product Description
Human CSTB (NP_000091, 1 - 98 a.a.) full length recombinant protein with His tag expressed in Escherichia coli expression system.
Host
Escherichia coli
Theoretical MW (kDa)
13
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU/ug
Activity
The IC50 value is < 3.0 nM. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
3 ug SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, 50 mM NaCl, pH 8.0
Storage Instruction
Store at 4°C for one week. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — CSTB
Entrez GeneID
1476Protein Accession#
P04080Gene Name
CSTB
Gene Alias
CST6, EPM1, PME, STFB
Gene Description
cystatin B (stefin B)
Gene Ontology
HyperlinkGene Summary
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq
Other Designations
CPI-B|cystatin B|liver thiol proteinase inhibitor|stefin B
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