Human NME1 (NP_000260, 1 - 152 a.a.) full length recombinant protein expressed in Escherichia coli expression system
Host:
Escherichia coli
Theoretical MW (kDa):
17.1
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Purity:
> 90% by SDS-PAGE
Endotoxin Level:
< 1 EU/ug
Activity:
Specific activity is > 1,200 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25°C in a couple system with PK/LDH.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
3 ug SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM Tris-HCl, 1 mM DTT, pH 7.5 (10% glycerol)
Storage Instruction:
Store at 4°C for one week. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq