PGK1 (Human) Recombinant Protein
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Specification
Product Description
Human PGK1 (NP_000282, 1 - 417 a.a.) full length recombinant protein with His tag expressed in Escherichia coli expression system.
Host
Escherichia coli
Theoretical MW (kDa)
46.8
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU/ug
Activity
Specific activity > 600 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37°C.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
3 ug SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, 1 mM DTT, pH 8.0 (10% glycerol)
Storage Instruction
Store at 4°C for one week. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — PGK1
Entrez GeneID
5230Protein Accession#
P00558Gene Name
PGK1
Gene Alias
MGC117307, MGC142128, MGC8947, MIG10, PGKA
Gene Description
phosphoglycerate kinase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq
Other Designations
OTTHUMP00000023595|cell migration-inducing gene 10 protein|primer recognition protein 2
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Glycolysis / Gluconeogenesis
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