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Last updated: 2022/5/22

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GPI Human Recombinant Protein 

  • Catalog # : P6936
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  • Specification
  • Product Description:
  • Human GPI (NP_000166, 1 - 558 a.a.) full length recombinant protein with His tag expressed in Escherichia coli expression system
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 65.3
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • > 95% by SDS-PAGE
  • Endotoxin Level:
  • < 1 EU/ug
  • Activity:
  • Specific activity is > 400 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will convert 1.0 umole of D-Fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 37°C.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6936
    3 ug SDS-PAGE under reducing condition and visualized by coomassie blue stain.
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM Tris-HCl, 1 mM DTT, pH 8.0 (10% glycerol)
  • Storage Instruction:
  • Store at 4°C for one week. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • SDS-PAGE
  • Application Image
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 2821
  • Gene Name:
  • GPI
  • Gene Alias:
  • AMF,GNPI,NLK,PGI,PHI,SA-36
  • Gene Description:
  • glucose phosphate isomerase
  • Gene Summary:
  • This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq
  • Other Designations:
  • autocrine motility factor,glucose-6-phosphate isomerase,hexose monophosphate isomerase,hexosephosphate isomerase,neuroleukin,oxoisomerase,phosphoglucose isomerase,phosphohexomutase,phosphohexose isomerase,phosphosaccharomutase,sperm antigen-36
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