Products

Product Description

Human GPI (NP_000166, 1 - 558 a.a.) full length recombinant protein with His tag expressed in Escherichia coli expression system.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Full-Length Protein,Full-Length Proteins,Full-Length,Full Length,FullLength

Host

Escherichia coli

Theoretical MW (kDa)

65.3

Form

Liquid

Preparation Method

Escherichia coli expression system

Purity

> 95% by SDS-PAGE

Endotoxin Level

< 1 EU/ug

Activity

Specific activity is > 400 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will convert 1.0 umole of D-Fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 37°C.

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue

3 ug SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Recommend Usage

SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

In 20 mM Tris-HCl, 1 mM DTT, pH 8.0 (10% glycerol)

Storage Instruction

Store at 4°C for one week. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.