Human ALPP (P05187, 23 a.a. - 250 a.a.) partial length recombinant protein expressed in Baculovirus expression system.
Host:
Viruses
Theoretical MW (kDa):
53.9
Form:
Liquid
Preparation Method:
Baculovirus expression system
Purity:
> 95% by SDS-PAGE
Endotoxin Level:
< 1.0 EU/μg
Activity:
Specific activity is > 2,500 units/mg, and is defined as the amount of enzyme that hydrolyzes 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (10% glycerol)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq