Human Glucose-6-phosphate 1-dehydrogenase recombinant protein expressed in Hi-5 cells.
Host:
Human
Theoretical MW (kDa):
61.4
Form:
Liquid
Preparation Method:
Insect cell (Hi-5) expression system
Purity:
> 95% pure
Activity:
Specific activity is > 7 units/mL obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of bet.
Storage Buffer:
In 20 mM Tris-HCl buffer, 200 mM NaCl, 2 mM EDTA, pH 8.0 (20% glycerol, 2 mM DTT, 0.1mM PMSF). Please Note: Buffer may change with new Lots.
Storage Instruction:
Store at 4°C for short term storage. For long term storage store at -20°C. For long term storage it is recommend to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing.