Carbonic anhydrase (Escherichia coli) Recombinant protein
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Specification
Product Description
Escherichia coli Carbonic anhydrase recombinant protein expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
27
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Activity
Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37 °C.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (10% glycerol, 1 mM DTT).
Storage Instruction
Store at 4°C for 1-2 weeks. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
Enzyme Activity
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