Glucokinase (Escherichia coli) Recombinant protein
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Specification
Product Description
Escherichia coli Glucokinase recombinant protein expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
37.1
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Activity
Specific activity is > 70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of B.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl buffer, 0.15 M NaCl, pH8.0 (10% glycerol).
Storage Instruction
Store at 4°C for 1-2 weeks. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
Enzyme Activity
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