Human TIMP1 recombinant protein expressed in Escherichia coli.
Host:
Human
Theoretical MW (kDa):
20.6
Form:
Lyophilized
Preparation Method:
Escherichia coli expression system
Purity:
> 95% pure
Endotoxin Level:
Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
Activity:
TIMP1 activity was measured by its ability to inhibit human MMP-1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP-1 concentration of approximately 0.5 ug/mL, when using an MMP-1 con.
Storage Instruction:
Store the lyophilized protein at -20°C for long term. Working aliquots stored with a carrier protein are stable for 3 months at -20°C. Aliquot to avoid repeated freezing and thawing.