ARG1 (Human) Recombinant Protein
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Specification
Product Description
Human Arginase 1/ARG1 protein (NP_000036, 1 a.a.-322 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
35.8
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 85% by SDS-PAGE
Activity
Specific activity is > 150,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of arginine to urea per minute at pH 10.5 at 37°C.
Quality Control Testing
SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, 100 mM NaCl, pH 8.0 (20% glycerol, 2 mM DTT)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — ARG1
Entrez GeneID
383Protein Accession#
P05089Gene Name
ARG1
Gene Alias
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Gene Description
arginase, liver
Gene Ontology
HyperlinkGene Summary
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq
Other Designations
A-I|OTTHUMP00000017209|arginase, type I
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