GCK (Human) Recombinant Protein
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Specification
Product Description
Human Hexokinase 4 protein (NP_000153, 1 a.a.-465 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
54.3
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU/ug
Activity
Specific activity is > 2,000 pmol/min/ug. One unit will convert 1 pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37°C.
Quality Control Testing
15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.
15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, pH 8.0 (10% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — GCK
Entrez GeneID
2645Protein Accession#
P35557Gene Name
GCK
Gene Alias
GK, GLK, HHF3, HK4, HKIV, HXKP, MODY2
Gene Description
glucokinase (hexokinase 4)
Gene Ontology
HyperlinkGene Summary
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq
Other Designations
ATP:D-hexose 6-phosphotransferase|OTTHUMP00000024521|OTTHUMP00000159308|glucokinase|hexokinase D, pancreatic isozyme
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