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Last updated: 2022/8/14

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HAO1 (Human) Recombinant Protein BioActive

  • Catalog # : P6832
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  • Specification
  • Product Description:
  • Human Hydroxyacid Oxidase-1/HAO-1 protein (NP_060015, 1 a.a.-370 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 45
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • > 95% by SDS-PAGE
  • Endotoxin Level:
  • < 1 EU/ug
  • Activity:
  • Specific activity is > 3,000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25°C.
  • Quality Control Testing:
  • 15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.

    QC Testing of P6832
    15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM Tris-HCl, 0.5M NaCl, pH 8.0 (20% glycerol)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • SDS-PAGE
  • Application Image
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • HAO1
  • Gene Alias:
  • GOX,GOX1,HAOX1,MGC142225,MGC142227
  • Gene Description:
  • hydroxyacid oxidase (glycolate oxidase) 1
  • Gene Summary:
  • This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq
  • Other Designations:
  • (S)-2-hydroxy-acid oxidase,OTTHUMP00000030231,glycolate oxidase,hydroxyacid oxidase 1
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