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Last updated: 2022/5/22

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ADK (Human) Recombinant Protein BioActive

  • Catalog # : P6828
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  • Specification
  • Product Description:
  • Human Adenosine Kinase/ADK protein (NP_006712, 22 a.a.-362 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 40.5
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • > 95% by SDS-PAGE
  • Activity:
  • Specific activity is > 30 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37°C in a couple system with PK and LDH.
  • Quality Control Testing:
  • 15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.

    QC Testing of P6828
    15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM Tris-HCl, 50 mM NaCl, pH 8.0 (20% glycerol, 1 mM DTT, 1 mM EDTA)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • SDS-PAGE
  • Application Image
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 132
  • Gene Name:
  • ADK
  • Gene Alias:
  • AK
  • Gene Description:
  • adenosine kinase
  • Gene Summary:
  • This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019864,OTTHUMP00000019865,adenosine 5'-phosphotransferase
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