Products

Product Description

Mouse Enolase 1/Non-neural enolase protein (NP_075608, 1 a.a.-434 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Full-Length Protein,Full-Length Proteins,Full-Length,Full Length,FullLength

Host

Escherichia coli

Theoretical MW (kDa)

49.5

Form

Liquid

Preparation Method

Escherichia coli expression system

Purity

> 90% by SDS-PAGE

Activity

Specific activity is > 6000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37°C in a couple system with PK and LDH.

Quality Control Testing

15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.

15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Recommend Usage

SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

In 20 mM Tris-HCl, 0.1 M NaCl, pH 8.5 (40% glycerol)

Storage Instruction

Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.