FBP1 (Human) Recombinant Protein
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Specification
Product Description
Human FBP1 (NP_000498.2, 1 a.a. - 338 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
39
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% by SDS-PAGE
Activity
Specific activity is > 7,000 pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37°C.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
15% SDS-PAGE Stained with Coomassie Blue.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, 1 mM DTT, pH 8.0 (10% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — FBP1
Entrez GeneID
2203Protein Accession#
P09467Gene Name
FBP1
Gene Alias
FBP
Gene Description
fructose-1,6-bisphosphatase 1
Gene Ontology
HyperlinkGene Summary
Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq
Other Designations
OTTHUMP00000021694|fructose-bisphosphatase 1|growth-inhibiting protein 17
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