Human FBP1 (NP_000498.2, 1 a.a. - 338 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
39
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Purity:
> 90% by SDS-PAGE
Activity:
Specific activity is > 7,000 pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37°C.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
15% SDS-PAGE Stained with Coomassie Blue.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM Tris-HCl, 1 mM DTT, pH 8.0 (10% glycerol)
Storage Instruction:
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.
Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq
Other Designations:
OTTHUMP00000021694,fructose-bisphosphatase 1,growth-inhibiting protein 17