GLO1 (Human) Recombinant Protein
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Specification
Product Description
Human GLO1 (NP_001209.1, 25 a.a. - 301 a.a.) partial recombinant protein with His tag expressed in Baculovirus expression system.
Host
Viruses
Theoretical MW (kDa)
20.7
Form
Liquid
Preparation Method
Baculovirus expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1 EU/ug
Activity
Specific activity is > 400 unit/mg. One unit will form 1.0 umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25°C.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
15% SDS-PAGE Stained with Coomassie Blue.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, 1 mM DTT, pH 8.0 (10% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — GLO1
Entrez GeneID
2739Protein Accession#
Q04760Gene Name
GLO1
Gene Alias
GLOD1, GLYI
Gene Description
glyoxalase I
Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq
Other Designations
OTTHUMP00000016339|glyoxalase domain containing 1|lactoyl glutathione lyase|lactoylglutathione lyase
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