Human GLO1 (NP_001209.1, 25 a.a. - 301 a.a.) partial recombinant protein with His tag expressed in Baculovirus expression system.
Host:
Viruses
Theoretical MW (kDa):
20.7
Form:
Liquid
Preparation Method:
Baculovirus expression system
Purity:
> 90% by SDS-PAGE
Endotoxin Level:
< 1 EU/ug
Activity:
Specific activity is > 400 unit/mg. One unit will form 1.0 umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25°C.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
15% SDS-PAGE Stained with Coomassie Blue.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM Tris-HCl, 1 mM DTT, pH 8.0 (10% glycerol)
Storage Instruction:
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq