CA12 (Human) Recombinant Protein
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Specification
Product Description
Human CA12 (NP_940986, 1 a.a. - 262 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
31.94
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1 EU/ug
Activity
Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37°C.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
SDS-PAGE Stained with Coomassie Blue.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — CA12
Entrez GeneID
771Protein Accession#
O43570Gene Name
CA12
Gene Alias
CAXII, FLJ20151, HsT18816
Gene Description
carbonic anhydrase XII
Omim ID
603263Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
Other Designations
carbonic dehydratase
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