Human CD24 (P25063, 27 a.a. - 59 a.a.) partial recombinant protein with mFc tag at C-terminal expressed in Expi293 cells.
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell (Expi293) expression system
Purification:
Protein A purification
Purity:
> 95% by Tris-Bis PAGE
Endotoxin Level:
< 1.0 EU/ug of the protein by LAL method.
Activity:
Immobilized CD24 (Human) Recombinant Protein (Cat # P6794), mFc Tag at 0.2 ug/mL (100 uL/Well). Dose response curve for Anti-CD24 Ab. with the ED50 of 0.19 ug/mL determined by ELISA.
Quality Control Testing:
Tris-Bis PAGE with Coomassie Blue
CD24 (Human) Recombinant Protein (Cat # P6794) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4.
Storage Instruction:
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Aliquot to avoid repeated freezing and thawing.