SIGLEC15 (Human) Recombinant Protein
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Specification
Product Description
Human SIGLEC15 (NP_998767.1, 20 a.a. - 263 a.a.) partial recombinant protein with Fc, 6x His tag at C-terminal expressed in HEK293 cells.
Host
Human
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-sepharose purification
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug of the protein by LAL method.
Activity
Measured by the ability to inhibit Anti-CD3-induced proliferation of jurkat cells. The ED50 for this effect is 1.8-7.2 ug/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
SIGLEC15 (Human) Recombinant Protein (Cat # P6792) was determined by SDS-PAGE with Coomassie Blue, showing a band at 60 kDa.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, 300 mM NaCl, pH 7.4.
Storage Instruction
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — SIGLEC15
Entrez GeneID
284266Protein Accession#
NP_998767.1Gene Name
SIGLEC15
Gene Alias
CD33L3, HsT1361, SIGLEC-15
Gene Description
sialic acid binding Ig-like lectin 15
Gene Ontology
HyperlinkOther Designations
CD33 antigen-like 3|CD33 molecule-like 3
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